BrdU 抗体
(5 references)Quick Overview for BrdU 抗体 (ABIN93883)
抗原
See all BrdU 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-BrdU Purified
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特异性
- The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It reacts also specifically with 5-bromouridine (BrU), and it does not cross-react with EdU.
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- 5-bromodeoxyuridine conjugated with hemocyanine.
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亚型
- IgG1
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anti-Bromodeoxyuridine (BrdU) antibody
BrdU FACS, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal Bu20a unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), WB 宿主: 小鼠 Monoclonal A7 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, FACS, IF, WB, ELISA, FM, DB 宿主: 小鼠 Monoclonal 29G6-E8 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 小鼠 Monoclonal BRD469 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), ICC, IF (cc), IF (p), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 小鼠 Monoclonal 4B6C12 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 兔 Monoclonal BRD2888R unconjugated Recombinant Antibody
anti-Bromodeoxyuridine (BrdU) antibody (PE)BrdU ICFC 宿主: 小鼠 Monoclonal Bu20a PE
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应用备注
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Flow cytometry: Recommended dilution: 1-2 μg/mL. Intracellular staining.
Immunocytochemistry: Recommended dilution: 2 μg/mL.
Immunohistochemistry (paraffin sections): Excellent. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "4D Genome Rewiring during Oncogene-Induced and Replicative Senescence." in: Molecular cell, Vol. 78, Issue 3, pp. 522-538.e9, (2020) (PubMed).
: "Premature mitotic entry induced by ATR inhibition potentiates olaparib inhibition-mediated genomic instability, inflammatory signaling, and cytotoxicity in BRCA2-deficient cancer cells." in: Molecular oncology, Vol. 13, Issue 11, pp. 2422-2440, (2020) (PubMed).
: "Cell proliferation in the hippocampus and in the heart is modified by exposure to repeated stress and treatment with memantine." in: Journal of psychiatric research, Vol. 46, Issue 4, pp. 526-32, (2012) (PubMed).
: "Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription." in: European journal of cell biology, Vol. 79, Issue 3, pp. 202-7, (2000) (PubMed).
: "Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects." in: The International journal of developmental biology, Vol. 42, Issue 5, pp. 737-40, (1998) (PubMed).
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: "4D Genome Rewiring during Oncogene-Induced and Replicative Senescence." in: Molecular cell, Vol. 78, Issue 3, pp. 522-538.e9, (2020) (PubMed).
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- BrdU (Bromodeoxyuridine (BrdU))
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别名
- BrdU
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物质类
- Chemical
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背景
- Bromodexyuridine (BrdU) is a thymidine analog, which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.,5-bromodeoxyuridine
抗原
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